Huachenyang (Shenzhen) Technology Co., Ltd
Create China's high-end biological sample collection and preservation medical products, and create an industry benchmark for microbial sample collection and preservation products!
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OEM PCR Nucleic Acid Purification Kit For Laboratory Detection
PCR Detection Swab Kit Nucleic Acid Purification Kit DNA Laboratory Detection Kit
product description:
HUACHENYANG DNA Extraction Kit provides a fast and efficient magnetic bead method to purify and extract DNA (including genomic, mitochondrial and viral DNA) from preserved tissues, saliva, body fluids, as well as from oral cavity, cervix, skin cells, bacterial cells, etc. Biological samples can be stored at room temperature for up to 30 days in our proprietary storage buffer before processing without significant loss of DNA yield or quality (more than 30 days if stored frozen) No phenol/ Chloroform extraction or alcohol precipitation yields high-quality genomic DNA within 15 minutes, with an average DNA yield of 8 μg per buccal swab. Purified DNA, approximately 20-30 kb, suitable for downstream applications such as PCR or other enzymatic reactions
Description:
The main components are magnetic beads, proteinase K, sodium chloride. Magnesium Chloride. Potassium chloride, Tris, etc.
feature:
Efficient, monospecific extraction of DNA to maximize removal of impurity proteins and other organic compounds from cells. The extracted DNA has large fragments, high purity, and stable and reliable quality.
| model | CY-F006-10 (50 preps-cells) |
| Magnetic beads | 0.8mL |
| Proteinase K solvent | 0.8mL |
| Protease lyophilized powder | 16mg |
| Reagent bottle size/volume1 | CY4/60mL |
| Reagent bottle size/volume2 | CY5/15mL |
Product Usage:
For purification and isolation of DNA (including genomic, mitochondrial, bacterial, parasitic and viral DNA) from tissues, saliva, body fluids and oral cavity, cervix, skin cells, bacterial cells, tissue, swabs, cerebrospinal fluid, body fluids, washed urine cells )
Product Instructions:
The magnetic bead material specifically adsorbs and dissociates from nucleic acid under different salt concentrations and acid-base conditions, separates nucleic acid and other components in the lysed cells by the transfer magnetic bead method, and then obtains the magnetic bead-free extract by washing and elution- -- Nucleic acids.
The first step - lysis, use lysis solution to destroy the cellular structure of animal and plant cells, so that the DNA is fully released
The second step—binding, adding magnetic bead binding solution to specifically adsorb DNA, so that DNA can be fully combined with magnetic beads;
The third step------washing, using detergent, after multiple washings, fully remove other residual impurities;
The fourth step - elution, use the eluent to dissociate the magnetic beads and DNA to obtain a higher-quality DNA solution.
Storage conditions and validity period:
1. The product should be used in an environment that is clean and hygienic, avoids pollution, and has a good use environment.
suitable temperature.
2. Store at room temperature. Proteinase K and magnetic beads can be stored longer at 2-8°C.
3. Product shelf life: 12 months